Advanced Coursework of Biomedical Sciences

Course Instructor

Hiromi Chayama
Center for Medical Specialist Graduate Education and Research, Graduate School of Biomedical and Health Sciences, Hiroshima University
PHS: 3540, E-mail: habe@hiroshima-u.ac.jp
Profiles of Faculty and Research Scholars

Over view

The course-work will focus on understanding of both the fundamental principles and techniques of molecular biology. Students will study nucleic acid structure, molecular genetics, and the biochemistry of mRNA transcription and protein synthesis. One mini project comprises five linked laboratory practices that introduces students mRNA and protein expression techniques using cultured mammalian cells with or without drug treatment. Students will learn about cell culturing, measurement of mRNA expression levels and protein expression levels. Based on this foundation, students should become able to design and perform experiments using proper assay controls and analyze data after completing this course-work.

Research project in this coursework

Blood cell-derived cell lines are differentiated by adding drugs, and the differentiated cells are observed by confocal laser microscopy or fluorescence microscopy, and the expressions of cell surface markers are analyzed by flow cytometry.

Student Evaluation Method

Reports

Language

Japanese/English

*The schedule is subject to change for various reasons.
*Please let me know if you will be absent.

Contents

The course is offered in the second semester. Please refer to the syllabus for the schedule and other information.

Practice #DateTopicObjectives
1
  • Friday
    9:30-12:30
 Cell Culturing and Induction of differentiation by drug addition Students will culture THP-1 and induce THP-1 cell differentiation by adding drugs.
2
  • Friday
    9:30-12:30
 Immunofluorescence Staining of cells, part1 Students will introduce the fluorescent protein gene into cultured cells by the lipofection method.
3
  • Friday
    9:30-12:30
 Immunofluorescence Staining of cells, part2 Students will stain cells with fluorescent dye-labeled antibodies
4
  • Friday
    9:30-12:30
 Immunofluorescence Staining of cells, part3 Students will observe with a confocal laser microscope or fluorescence microscope.
5
  • Friday
    9:30-12:30
 Cell surface antigen analysis by flow cytometry, part1 Students will stain the differentiated cells with antibodies against cell surface markers.
6
  • Thursday
    9:30-12:30
 Cell surface antigen analysis by flow cytometry, part2 Analysis of cell surface markers by flow cytometry
7
  • Friday
    9:30-12:30
 Cell surface antigen analysis by flow cytometry, part3 Students will analyze fluocytometry data using FlowJo software.
8
  • Friday
    9:30-12:30
 Data analysis Students will visualize the results and evaluate the experimental results.
  • Practice #
    1
    Date
    Friday 9:30-12:30
    Topic
    Cell Culturing and Induction of differentiation by drug addition
    Objectives
    Students will culture THP-1 and induce THP-1 cell differentiation by adding drugs.
  • Practice #
    2
    Date
    Friday 9:30-12:30
    Topic
    Immunofluorescence Staining of cells, part1
    Objectives
    Students will introduce the fluorescent protein gene into cultured cells by the lipofection method.
  • Practice #
    3
    Date
    Friday 9:30-12:30
    Topic
    Immunofluorescence Staining of cells, part2
    Objectives
    Students will stain cells with fluorescent dye-labeled antibodies
  • Practice #
    4
    Date
    Friday 9:30-12:30
    Topic
    Immunofluorescence Staining of cells, part3
    Objectives
    Students will observe with a confocal laser microscope or fluorescence microscope.
  • Practice #
    5
    Date
    Friday 9:30-12:30
    Topic
    Cell surface antigen analysis by flow cytometry, part1
    Objectives
    Students will stain the differentiated cells with antibodies against cell surface markers.
  • Practice #
    6
    Date
    Thursday 9:30-12:30
    Topic
    Cell surface antigen analysis by flow cytometry, part2
    Objectives
    Analysis of cell surface markers by FACSCanto2
  • Practice #
    7
    Date
    Friday 9:30-12:30
    Topic
    Cell surface antigen analysis by flow cytometry, part3
    Objectives
    Students will analyze fluocytometry data using FlowJo software.
  • Practice #
    8
    Date
    Friday 9:30-12:30
    Topic
    Data analysis
    Objectives
    Students will visualize the results and evaluate the experimental results.
© Center for Medical Specialist Graduate Education and Research,
Graduate School of Biomedical and Health Sciences, Hiroshima University