Start-up Coursework of Biomedical Sciences

Course Instructor

Hiromi Chayama
Center for Medical Specialist Graduate Education and Research, Graduate School of Biomedical and Health Sciences, Hiroshima University
PHS: 3540, E-mail: habe@hiroshima-u.ac.jp
Profiles of Faculty and Research Scholars

Over view

Students will perform Cell culturing, nucleic acid and protein level expression analyses. Through this coursework, students will learn the basics of molecular biological experiments and understand the mechanisms of expression regulation from genomes to proteins that function in the body.

Student Evaluation Method

Reports

Language

Japanese/English

*The schedule is subject to change for various reasons.
*Please let me know if you will be absent.

Contents

The first and second semesters are offered. In the second semester, only Group A is available. Please refer to the syllabus for schedule details.

Practice #DateTopicObjectives
1
  • A Group Wednesday
    9:30-12:30
  • B Group Friday
    9:30-12:30
 Review of molecular biology, Practice of cell culturing.Students will understand DNA,RNA and protein structures, molecular genetics and the biochemistry of transcription and protein synthesis. In addition, they will do cell culturing.
2
  • A Group Wednesday
    9:30-12:30
  • B Group Friday
    9:30-12:30
Transfection DNA Plasmid into Mammalian CellsStudents can carry out basic cell-culture and transfection techniques properly and safely.
3
  • A Group Tuesday
    9:30-15:30
  • B Group Thursday
    9:30-15:30
Protein gel electrophoresis part1Students will learn about principle and technique of protein gel electrophoresis using SDS-PAGE. In addition, they will set up gels and perform western blots.
4
  • A Group Wednesday
    9:30-12:30
  • B Group Friday
    9:30-12:30
Protein gel electrophoresis part2Students will detect targeted protein expression using ECL reagents with the ChemiDoc imaging system.
5
  • A Group Wednesday
    9:30-12:30
  • B Group Friday
    9:30-12:30
Purification of nucleic acidStudents will learn about principle and technique of nucleic acid purification, reverse transcription and cDNA synthesis.
6
  • A Group Tuesday
    9:30-12:30
  • B Group Thursday
    9:30-12:30
PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 1Students will search for primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis. In addition, Students will learn about principle and technique of mRNA expression level quantification by real time PCR.
7
  • A Group Wednesday
    9:30-12:30
  • B Group Friday
    9:30-12:30
PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 2
8
  • A Group Wednesday
    9:30-12:30
  • B Group Friday
    9:30-12:30
Data analysis and review of the classStudents will analyze data by T-TEST and report their image data by western blots.
  • Practice #
    1
    Date
    A Group Wednesday 9:30-12:30
    B Group Friday 9:30-12:30
    Topic
    Review of molecular biology, Practice of cell culturing.
    Objectives
    Students will understand DNA,RNA and protein structures, molecular genetics and the biochemistry of transcription and protein synthesis. In addition, they will do cell culturing.
  • Practice #
    2
    Date
    A Group Wednesday 9:30-12:30
    B Group Friday 9:30-12:30
    Topic
    Transfection DNA Plasmid into Mammalian Cells
    Objectives
    Students can carry out basic cell-culture and transfection techniques properly and safely.
  • Practice #
    3
    Date
    A Group Tuesday 9:30-15:30
    B Group Thursday 9:30-15:30
    Topic
    Protein gel electrophoresis part1
    Objectives
    Students will learn about principle and technique of protein gel electrophoresis using SDS-PAGE. In addition, they will set up gels and perform western blots.
  • Practice #
    4
    Date
    A Group Wednesday 9:30-12:30
    B Group Friday 9:30-12:30
    Topic
    Protein gel electrophoresis part2
    Objectives
    Students will detect targeted protein expression using ECL reagents with the ChemiDoc imaging system.
  • Practice #
    5
    Date
    A Group Wednesday 9:30-12:30
    B Group Friday 9:30-12:30
    Topic
    Purification of nucleic acid
    Objectives
    Students will learn about principle and technique of nucleic acid purification, reverse transcription and cDNA synthesis.
  • Practice #
    6
    Date
    A Group Tuesday 9:30-12:30
    B Group Thursday 9:30-12:30
    Topic
    PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 1
    Objectives
    Students will search for primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis. In addition, Students will learn about principle and technique of mRNA expression level quantification by real time PCR.
  • Practice #
    7
    Date
    A Group Wednesday 9:30-12:30
    B Group Friday 9:30-12:30
    Topic
    PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 2
    Objectives
    Students will search for primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis. In addition, Students will learn about principle and technique of mRNA expression level quantification by real time PCR.
  • Practice #
    8
    Date
    A Group Wednesday 9:30-12:30
    B Group Friday 9:30-12:30
    Topic
    Data analysis and review of the class
    Objectives
    Students will analyze data by T-TEST and report their image data by western blots.
© Center for Medical Specialist Graduate Education and Research,
Graduate School of Biomedical and Health Sciences, Hiroshima University