Start-up Coursework of Biomedical Sciences

2021 Semester Syllabus

To prevent the spread of COVID-19, classes of the university from April will be implemented as "Policy regarding the classes of the 2020 Spring Semester (1st and 2nd term)".
Please check the latest information on this website and "Momiji".

Course Instructor

Hiromi Chayama
Center for Medical Specialist Graduate Education and Research, Graduate School of Biomedical and Health Sciences, Hiroshima University
PHS: 3540, E-mail: habe@hiroshima-u.ac.jp

Over view

Students will perform Cell culturing, nucleic acid and protein level expression analyses. Through this coursework, students will learn the basics of molecular biological experiments and understand the mechanisms of expression regulation from genomes to proteins that function in the body.

Student Evaluation Method

Attendance rate (a report on an absent topic will count as attendance).

Language

Japanese/English

*The schedule is subject to change for various reasons.
*Please let me know if you will be absent.

2021 1st Semester Syllabus

Practice #DateTopicObjectivesPlaceNote
1
  • A Group Wednesday
    13:00-16:00
  • B Group Friday
    9:00-12:00
Review of molecular biology, Practice of cell culturing.Students will understand DNA,RNA and protein structures, molecular genetics and the biochemistry of transcription and protein synthesis. In addition, they will do cell culturing.Building A, 6th floor A634You are required to provide your lab coat and writing implements.
2
  • A Group Wednesday
    13:00-16:00
  • B Group Friday
    9:00-12:00
Transfection DNA Plasmid into Mammalian CellsStudents can carry out basic cell-culture and transfection techniques properly and safely.Building A, 6th floor A634You are required to provide your lab coat and writing implements.
3
  • A Group Wednesday
    13:00-16:00
  • B Group Friday
    9:00-12:00
Purification of nucleic acidStudents will learn about principle and technique of nucleic acid purification, reverse transcription and cDNA synthesis.Building A, 6th floor A634You are required to provide your lab coat and writing implements.
4
  • A Group Wednesday
    13:00-16:00
  • B Group Friday
    9:00-12:00
PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 1Students will search for primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis. In addition, Students will learn about principle and technique of mRNA expression level quantification by real time PCR.Building A, 6th floor A634You are required to provide your lab coat and writing implements.
5
  • A Group Wednesday
    13:00-16:00
  • B Group Friday
    9:00-12:00
PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 2
6
  • A Group Tuesday
    9:30-15:30
  • B Group Thursday
    9:30-15:30
Protein gel electrophoresis part1Students will learn about principle and technique of protein gel electrophoresis using SDS-PAGE. In addition, they will set up gels and perform western blots.Building A, 6th floor A634You are required to provide your lab coat and writing implements.
7
  • A Group Wednesday
    13:00-16:00
  • B Group Friday
    9:00-12:00
Protein gel electrophoresis part2Students will detect targeted protein expression using ECL reagents with the ChemiDoc imaging system.Building A, 6th floor A634You are required to provide your lab coat and writing implements.
8
  • A Group Wednesday
    13:00-16:00
  • B Group Friday
    9:00-12:00
Data analysis and review of the classStudents will analyze data by T-TEST and report their image data by western blots.Building A, 6th floor A634You are required to provide your laptop and writing implements.
  • Practice #
    1
    Date
    A Group Wednesday 13:00-16:00
    B Group Friday 9:00-12:00
    Topic
    Review of molecular biology, Practice of cell culturing.
    Objectives
    Students will understand DNA,RNA and protein structures, molecular genetics and the biochemistry of transcription and protein synthesis. In addition, they will do cell culturing.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    2
    Date
    A Group Wednesday 13:00-16:00
    B Group Friday 9:00-12:00
    Topic
    Transfection DNA Plasmid into Mammalian Cells
    Objectives
    Students can carry out basic cell-culture and transfection techniques properly and safely.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    3
    Date
    A Group Wednesday 13:00-16:00
    B Group Friday 9:00-12:00
    Topic
    Purification of nucleic acid
    Objectives
    Students will learn about principle and technique of nucleic acid purification, reverse transcription and cDNA synthesis.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    4
    Date
    A Group Wednesday 13:00-16:00
    B Group Friday 9:00-12:00
    Topic
    PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 1
    Objectives
    Students will search for primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis. In addition, Students will learn about principle and technique of mRNA expression level quantification by real time PCR.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    5
    Date
    A Group Wednesday 13:00-16:00
    B Group Friday 9:00-12:00
    Topic
    PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 2
    Objectives
    Students will search for primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis. In addition, Students will learn about principle and technique of mRNA expression level quantification by real time PCR.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    6
    Date
    A Group Tuesday 9:30-15:30
    B Group Thursday 9:30-15:30
    Topic
    Protein gel electrophoresis part1
    Objectives
    Students will learn about principle and technique of protein gel electrophoresis using SDS-PAGE. In addition, they will set up gels and perform western blots.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    7
    Date
    A Group Wednesday 13:00-16:00
    B Group Friday 9:00-12:00
    Topic
    Protein gel electrophoresis part2
    Objectives
    Students will detect targeted protein expression using ECL reagents with the ChemiDoc imaging system.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    8
    Date
    A Group Wednesday 13:00-16:00
    B Group Friday 9:00-12:00
    Topic
    Data analysis and review of the class
    Objectives
    Students will analyze data by T-TEST and report their image data by western blots.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your laptop and writing implements.

2021 2nd Semester Syllabus

Practice #DateTopicObjectivesPlaceNote
1
  • Wednesday
    13:00-16:00
Nucleic acids, proteins, and cells Students will review the basics of molecular biology Building A, 6th floor A634 You are required to provide your lab coat and writing implements.
2
  • Wednesday
    13:00-16:00
Cell Culturing Students will learn to operate in the biological safety cabinet and carry out basic cell-culture properly and safely. Building A, 6th floor A634 You are required to provide your lab coat and writing implements.
3
  • Wednesday
    13:00-16:00
Nucleic acid extraction The student will purify total RNAs from the cells and determine the concentration and purity of total RNAs. Students will also carry out reverse transcription reactions to is carried out to synthesize cDNA using the obtained total RNAs. Building A, 6th floor A634 You are required to provide your lab coat and writing implements.
4
  • Wednesday
    13:00-16:00
Primer design, PCR and Agarose gel electrophoresis Students will design primers, amplify the target gene by PCR and confirm the specific gene amplification by agarose gel electrophoresis. Building A, 6th floor A634 You are required to provide your lab coat and writing implements.
5
  • Wednesday
    13:00-16:00
Real time PCR Students will quantify the expression levels of the target gene and endogenous control gene by real time PCR. Building A, 6th floor A634 You are required to provide your lab coat and writing implements.
6
  • Tuesday
    9:30-15:30
Western Blotting, Part 1 Students will detect the expressions of the target proteins by SDS-PAGE and western blotting. Building A, 6th floor A634 You are required to provide your lab coat and writing implements.
7
  • Wednesday
    13:00-16:00
Western Blotting, Part 2 Building A, 6th floor A634 You are required to provide your lab coat and writing implements.
8
  • Wednesday
    13:00-16:00
Data analysis Students will discuss the results of the mRNA and protein expression levels obtained in the experiment. Building A, 6th floor A634 You are required to provide your laptop and writing implements.
  • Practice #
    1
    Date
    Wednesday 13:00-16:00
    Topic
    Nucleic acids, proteins, and cells
    Objectives
    Students will review the basics of molecular biology
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    2
    Date
    Wednesday 13:00-16:00
    Topic
    Cell Culturing
    Objectives
    Students will learn to operate in the biological safety cabinet and carry out basic cell-culture properly and safely.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    3
    Date
    Wednesday 13:00-16:00
    Topic
    Nucleic acid extraction
    Objectives
    The student will purify total RNAs from the cells and determine the concentration and purity of total RNAs. Students will also carry out reverse transcription reactions to is carried out to synthesize cDNA using the obtained total RNAs.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    4
    Date
    Wednesday 13:00-16:00
    Topic
    Primer design, PCR and Agarose gel electrophoresis
    Objectives
    Students will design primers, amplify the target gene by PCR and confirm the specific gene amplification by agarose gel electrophoresis.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    5
    Date
    Wednesday 13:00-16:00
    Topic
    Real time PCR
    Objectives
    Students will quantify the expression levels of the target gene and endogenous control gene by real time PCR.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    6
    Date
    Tuesday 9:00-12:00
    Topic
    Western Blotting, Part 1
    Objectives
    Students will detect the expressions of the target proteins by SDS-PAGE and western blotting.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    7
    Date
    Wednesday 13:00-16:00
    Topic
    Western Blotting, Part 2
    Objectives
    Students will detect the expressions of the target proteins by SDS-PAGE and western blotting.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your lab coat and writing implements.
  • Practice #
    8
    Date
    Wednesday 13:00-16:00
    Topic
    Data analysis
    Objectives
    Students will discuss the results of the mRNA and protein expression levels obtained in the experiment.
    Place
    Building A, 6th floor A634
    Note
    You are required to provide your laptop and writing implements.
© Center for Medical Specialist Graduate Education and Research,
Graduate School of Biomedical and Health Sciences, Hiroshima University